| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210828 |
| Chromosome: | chromosome 2 |
| Location: | 6103811 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113200 | GLN1,NSG18 | (1 of 2) PTHR20852:SF50 - GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-1-RELATED; Glutamine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCATGTGTTCCCTCATGTAGGGGAGGCG |
| Internal bar code: | GGTTGGGGCGTATAGCCAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 97.4735 |
| LEAP-Seq n confirming: | 7176 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTATTCCTGCTCCCGAA |
| Suggested primer 2: | AAGGTTGTGTCCAGGTTTCG |