Insertion junction: LMJ.RY0402.210837_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.210837
Chromosome:chromosome 12
Location:8593107
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g547300 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTAGGCGTGGTCAAGAGCGTGTGTGACA
Internal bar code:CAGCCCTCTGGGACATCTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:765
LEAP-Seq percent confirming:99.2028
LEAP-Seq n confirming:1991
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCAAATAGCTGGGCAAC
Suggested primer 2:TGACGCTGAGCATGGAATAG