| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210849 |
| Chromosome: | chromosome 16 |
| Location: | 4441677 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670900 | SMM50 | (1 of 14) PF10294 - Lysine methyltransferase (Methyltransf_16); S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTCCCATTCCAGATGACGCAAGCCAA |
| Internal bar code: | CCCCTGCGGAGTCGCTGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 99.6685 |
| LEAP-Seq n confirming: | 902 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTGGTGTACGTGAGTG |
| Suggested primer 2: | CGCCAAATGAGAGAACCATT |