Insertion junction: LMJ.RY0402.210868_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210868
Chromosome:chromosome 14
Location:3824627
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632700 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTTAAGAGCAATAAAAAAACAGCTGCA
Internal bar code:TGCTCATATGGGTGAGAAAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1083
LEAP-Seq percent confirming:99.2647
LEAP-Seq n confirming:4185
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGCATTAGTGAGGCAA
Suggested primer 2:TTGTCTGCACTTCCGTTGAG