| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210869 |
| Chromosome: | chromosome 12 |
| Location: | 5619578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532151 | ERM7 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein | 5'UTR/5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTGCTGCTCTCGACGCGCCTGAAGCA |
| Internal bar code: | TATGGGAGCGCACGGGAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 90.4093 |
| LEAP-Seq n confirming: | 2187 |
| LEAP-Seq n nonconfirming: | 232 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGCTTTTGTTTCGGAC |
| Suggested primer 2: | ACGTACACGTAGGCCAGGAC |