Insertion junction: LMJ.RY0402.210872_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210872
Chromosome:chromosome 13
Location:3741350
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTCGCTTTCACTCCAACTTCCGGCGCA
Internal bar code:ACAGATAATTGTTGAATAAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:99.5066
LEAP-Seq n confirming:605
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTAGCTGAACTCCACCG
Suggested primer 2:AACATGCCTGGTTGTAAGCC