| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.210905 |
| Chromosome: | chromosome 14 |
| Location: | 1460505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617950 | CAM18 | Calmodulin-like protein; (1 of 5) PTHR23050//PTHR23050:SF90 - CALCIUM BINDING PROTEIN // CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCAAAAGAGATGACCTGGGACGTTGTAC |
| Internal bar code: | GAGGTATCTTCGATGGGAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.4645 |
| LEAP-Seq n confirming: | 3529 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCACACCTACCTATGCTG |
| Suggested primer 2: | TCCCATGATGAAGAGGAAGG |