| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210911 |
| Chromosome: | chromosome 3 |
| Location: | 4325266 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174900 | (1 of 5) PF00536 - SAM domain (Sterile alpha motif) (SAM_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCTGGTCAAGTTCCGGGACGAGCTGAC |
| Internal bar code: | CGCATGTGGACAGACGTGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 99.9471 |
| LEAP-Seq n confirming: | 1889 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTCCCTAACGTACCGCT |
| Suggested primer 2: | ACTCGCAGGCTGTCGTAGAT |