| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210969 |
| Chromosome: | chromosome 12 |
| Location: | 3161980 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499400 | CYN2,CYN18-2,CYN18B | (1 of 1) K12733 - peptidyl-prolyl cis-trans isomerase-like 1 (PPIL1); Cyclophilin 18-2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGCGGTTTTGGGCCCGTGTGAGGCAG |
| Internal bar code: | GGTTGGGCCTTGTGCCCCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1240 |
| LEAP-Seq percent confirming: | 97.5904 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCGAGCGTGCTCATGTC |
| Suggested primer 2: | ATGTACTTTTACGGGCGTCG |