| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.210989 |
| Chromosome: | chromosome 5 |
| Location: | 2095572 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234637 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCCTCGGTCGTGCTGCATTTAATCTG |
| Internal bar code: | CGTGGCACCCGGTTGCGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 98.8146 |
| LEAP-Seq n confirming: | 9336 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCTATGAGGTGCGAGGT |
| Suggested primer 2: | AGATATTTTGGGGACAGGGG |