| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.210991 |
| Chromosome: | chromosome 10 |
| Location: | 5800793 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461400 | (1 of 1) PTHR22957:SF212 - [PROTEIN ADP-RIBOSYLARGININE] HYDROLASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGCCGTGTCAGCGCGCCAGGAACTGA |
| Internal bar code: | ATACGCGTGAACGCTTCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 91.5254 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGCGGGTTAATTGTCT |
| Suggested primer 2: | GTGCGTACATGTGAGGTTGG |