Insertion junction: LMJ.RY0402.211001_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211001
Chromosome:chromosome 3
Location:8326663
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199000 PHOT,PHT1,PHOT1 (1 of 1) PTHR24351//PTHR24351:SF76 - RIBOSOMAL PROTEIN S6 KINASE // PROTEIN TWIN LOV 1; Phototropin 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGTGGGTGCTAGTGCAGGCGTGGGGA
Internal bar code:TGAATCGAAATGCGGAACGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:773
LEAP-Seq percent confirming:99.452
LEAP-Seq n confirming:23409
LEAP-Seq n nonconfirming:129
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:AGCATCAGTCTGCAATCCCT
Suggested primer 2:GGTGTGCAGTCCTGAGACAA