Insertion junction: LMJ.RY0402.211001_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211001
Chromosome:chromosome 3
Location:8326663
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199000 PHOT,PHT1,PHOT1 (1 of 1) PTHR24351//PTHR24351:SF76 - RIBOSOMAL PROTEIN S6 KINASE // PROTEIN TWIN LOV 1; Phototropin 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACGTGCTGTGATGTCCCTCCCTCACAC
Internal bar code:ACATGGCTTGTCCTCTGGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:938
LEAP-Seq percent confirming:99.5578
LEAP-Seq n confirming:6079
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGCATCAGTCTGCAATCCCT
Suggested primer 2:GGTGTGCAGTCCTGAGACAA