Insertion junction: LMJ.RY0402.211116_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211116
Chromosome:chromosome 3
Location:8143947
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g200600 KIN14A1,KIN14A-1,KIN14-4 Kinesin motor protein; (1 of 2) K10405 - kinesin family member C1 (KIFC1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTGCAGCCGTCATCGTCTTGGCTACTG
Internal bar code:TAGTGGTGCAACGCTCGAACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:875
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:474
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAACCCTACTGCACCAT
Suggested primer 2:GTTGACAGTGACACCGATGG