Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.211131 |
Chromosome: | chromosome 9 |
Location: | 6560836 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g408051 | (1 of 2) K15032 - mTERF domain-containing protein, mitochondrial (MTERFD) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGGAGTTGTAGCCTGTGGGGGGCCAG |
Internal bar code: | GAGTTCTGGGTCACGTCCCTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 948 |
LEAP-Seq percent confirming: | 98.6535 |
LEAP-Seq n confirming: | 15679 |
LEAP-Seq n nonconfirming: | 214 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTGGGAACCTGGGAATAC |
Suggested primer 2: | CCATGTTGCTGACTACCCCT |