| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211142 |
| Chromosome: | chromosome 1 |
| Location: | 387520 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002400 | TGL1 | (1 of 1) 3.1.1.3//3.1.1.73 - Triacylglycerol lipase / Triglyceride lipase // Feruloyl esterase / Hydroxycinnamoyl esterase; Putative triacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGGAGCTGGACCCTGATCTTGATGCC |
| Internal bar code: | TTTTCGGTACGTTTCTCAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 99.3007 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACGCCTGATGTCTACTT |
| Suggested primer 2: | ACGCACACACACACACACAC |