Insertion junction: LMJ.RY0402.211150_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211150
Chromosome:chromosome 3
Location:3060058
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g164400 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTATTGATTGACCGGGGGTGGGCCCCG
Internal bar code:AATTGTTCTGCACTTACGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:96.9115
LEAP-Seq n confirming:3232
LEAP-Seq n nonconfirming:103
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGTGTTGTTGCATTGTG
Suggested primer 2:GAGACAGACTCGCCCTGTTC