| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211151 |
| Chromosome: | chromosome 3 |
| Location: | 1189185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149517 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGCGTACTCGGGAAGGTCGGGCTAACA |
| Internal bar code: | TCTGAGGACGCTACGACGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 89.1552 |
| LEAP-Seq n confirming: | 1488 |
| LEAP-Seq n nonconfirming: | 181 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTACCGCAAGGTCTACG |
| Suggested primer 2: | ACAAAGCCGGTGACATTTTC |