| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211163 |
| Chromosome: | chromosome 6 |
| Location: | 6498911 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACAGTCACGGGGACGCCGATGGCCAC |
| Internal bar code: | CGTCTGCGGACGGTAGATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 99.3108 |
| LEAP-Seq n confirming: | 4323 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGGGATTTGGGATTG |
| Suggested primer 2: | TGTTGCCTATCTACCCCAGG |