| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211175 |
| Chromosome: | chromosome 16 |
| Location: | 4395697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671300 | (1 of 3) K12041 - solute carrier family 9 (sodium/hydrogen exchanger), member 6/7 (SLC9A6_7, NHE6_7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAACTCACACGGATCACCACACGGTGG |
| Internal bar code: | GGTTGATGAAGCCGTTTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 66.8935 |
| LEAP-Seq n confirming: | 2457 |
| LEAP-Seq n nonconfirming: | 1216 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCTCGTCTACTGCAC |
| Suggested primer 2: | ACGCGGGTGGAAGTATGTAG |