| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211181 |
| Chromosome: | chromosome 3 |
| Location: | 5988328 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190550 | (1 of 1) PF08638 - Mediator complex subunit MED14 (Med14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTCAGTAGGACCCCACGCAATGCTGGC |
| Internal bar code: | GGAAGCCTAAGCAGCCGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 98.9819 |
| LEAP-Seq n confirming: | 1750 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGTTATGCCACATCCTT |
| Suggested primer 2: | GATTAGTTGGGACAAGGCCA |