Insertion junction: LMJ.RY0402.211197_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211197
Chromosome:chromosome 10
Location:6270642
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g464950 (1 of 1) K15201 - general transcription factor 3C polypeptide 3 (transcription factor C subunit 4) (GTP3C3, TFC4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTACAATGCAGTGGGGGAAGAGGAGGTG
Internal bar code:CATCTCAGCTAAGACGCGTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:99.5157
LEAP-Seq n confirming:411
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATAACGTGAACGGCAACCTC
Suggested primer 2:CGTGTGTGTGTGTGTGGTGT