Insertion junction: LMJ.RY0402.211198_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211198
Chromosome:chromosome 9
Location:6945792
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGGCAGAGTACAGGTAAAGTATGTCTCG
Internal bar code:AGGGGGACGAAGAGAGCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:41.9092
LEAP-Seq n confirming:1958
LEAP-Seq n nonconfirming:2714
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTGGAACATGGAGCATC
Suggested primer 2:AAGAGCAGGAGGAGGAGGAG