| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211200 |
| Chromosome: | chromosome 7 |
| Location: | 6122182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355650 | AMT1F,AMT6 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTACACGCTCGTCCAGCCCCACACACCG |
| Internal bar code: | CCGCAAAGTTTCGACTGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.6177 |
| LEAP-Seq n confirming: | 4430 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCCGTGTCTTCAGTTC |
| Suggested primer 2: | CGTTGAAGTTGAATCGCTCA |