| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211202 |
| Chromosome: | chromosome 1 |
| Location: | 6449411 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045825 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGGACATAGCGCATAGACGATGAGGT |
| Internal bar code: | AGAGGATCTTCGCTTTCCGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 98.9234 |
| LEAP-Seq n confirming: | 11577 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAGACGCATGAATGGCT |
| Suggested primer 2: | CTTGGTGAAGTCGAACAGCA |