| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211216 |
| Chromosome: | chromosome 13 |
| Location: | 1076881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568950 | (1 of 2) 3.6.1.19 - Nucleoside-triphosphate diphosphatase / Nucleoside-triphosphate pyrophosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCCTATAACAGCGCTCAACCATCAACC |
| Internal bar code: | TAAGCACCGGAGGGGCTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 98.9729 |
| LEAP-Seq n confirming: | 4240 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGATACTAGGGAGTCG |
| Suggested primer 2: | AGTGACCACAACTGACTGCG |