| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211223 |
| Chromosome: | chromosome 11 |
| Location: | 3231892 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479600 | MHX1,CAX6 | (1 of 1) K05849 - solute carrier family 8 (sodium/calcium exchanger) (SLC8A, NCX); putative Ca2+/Na+ exchanger | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCATCCCCTGCTGTACCCGTGGGACG |
| Internal bar code: | TCTCAATGCCAAACTTGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1127 |
| LEAP-Seq percent confirming: | 99.5854 |
| LEAP-Seq n confirming: | 4323 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCCCTCGCTGTGTTACC |
| Suggested primer 2: | AATCAATGCCTGGGTAGTGC |