| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211224 |
| Chromosome: | chromosome 16 |
| Location: | 2134887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658000 | Putative metallochaperone; (1 of 2) IPR003495//IPR027417 - CobW/HypB/UreG domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAAGGAAGTGGGGTTCTAGTTAAAGAAG |
| Internal bar code: | TTCAAACAAGCTTGCTTCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 98.9492 |
| LEAP-Seq n confirming: | 4049 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTAGTACCAGTGGCGGGC |
| Suggested primer 2: | GGTCACAGTCACAGTCGCAG |