Insertion junction: LMJ.RY0402.211226_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.211226
Chromosome:chromosome 9
Location:2531589
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g390957 (1 of 6) IPR001471//IPR016177 - AP2/ERF domain // DNA-binding domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACACCACACCACACCACGCAAGCACGCA
Internal bar code:CGAGGTAAAAGTCTTTTTTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:503
LEAP-Seq percent confirming:70.4063
LEAP-Seq n confirming:2755
LEAP-Seq n nonconfirming:1158
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGGGAGGAATACACGGAC
Suggested primer 2:TGCATTCGAACACGTACCAT