Insertion junction: LMJ.RY0402.211237_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211237
Chromosome:chromosome 7
Location:3954915
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g339500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGACGCGGCTGCGGGAATCGTGGAAAGG
Internal bar code:CGCACGGACATTTTCTCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:475
LEAP-Seq percent confirming:99.68
LEAP-Seq n confirming:4049
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTTCTGGAAGGACAAGC
Suggested primer 2:CCCACTTCCAAACTTCCAAA