| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211240 |
| Chromosome: | chromosome_7 |
| Location: | 2612502 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g330200 | RSP9 | Radial spoke protein 9 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAACCAAGGCCAGCGTGTGTGTGAAAGTGC |
| Internal bar code: | CGTCCTTCGGCTACATCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 99.4409 |
| LEAP-Seq n confirming: | 2846 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGGTGCCTAAAACAGAT |
| Suggested primer 2: | TCCTTCTCCTCCAGCTCGTA |