| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211248 |
| Chromosome: | chromosome 12 |
| Location: | 8344515 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549450 | (1 of 1) 3.7.1.5 - Acylpyruvate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCATGGTGCCACGTACGGTCCATGGGC |
| Internal bar code: | CGACTGCGCATCGAGTATGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.7712 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGGTGCATCGCTTCTTA |
| Suggested primer 2: | TACCAGAGGGGCCTAAAACC |