| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211251 |
| Chromosome: | chromosome 5 |
| Location: | 2235415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234660 | (1 of 4) IPR011016//IPR013083 - Zinc finger, RING-CH-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAATGGTGCCCAGCAGTTTCAAACGCA |
| Internal bar code: | GCCCACTGTCCGGGCACACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.4648 |
| LEAP-Seq n confirming: | 3345 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGAATCACAGATGCTGC |
| Suggested primer 2: | CATTGTGCAGCGTAGTCCAG |