| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211255 |
| Chromosome: | chromosome 8 |
| Location: | 499488 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358900 | (1 of 3) 2.4.2.8 - Hypoxanthine phosphoribosyltransferase / Transphosphoribosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTTGCTCCCCCCTCCCCGCGCTCCGC |
| Internal bar code: | GAACTAGTCCCAGAACGCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1163 |
| LEAP-Seq percent confirming: | 99.2265 |
| LEAP-Seq n confirming: | 12571 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCCCTCATTAGACTGCC |
| Suggested primer 2: | TTCCTATTAAAACCCGCACG |