| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211264 |
| Chromosome: | chromosome 7 |
| Location: | 2450230 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACGGCATAGCTGAGCAGCGAGCGTAGAC |
| Internal bar code: | CAGTACTCCATCGTGCCGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 82.3393 |
| LEAP-Seq n confirming: | 1422 |
| LEAP-Seq n nonconfirming: | 305 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGCGACCTGTGCTGACA |
| Suggested primer 2: | TGGACTGCATCAGAGTTTGC |