| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.211266 |
| Chromosome: | chromosome 12 |
| Location: | 6198720 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein 78; (1 of 7) PTHR11584:SF381 - PROTEIN PQN-80, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACCCCAGCCAGCCCGAGGTGCCCTTTA |
| Internal bar code: | GTGGTGTTCTCAGATCCGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 99.0884 |
| LEAP-Seq n confirming: | 1413 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTCTTCCGGAGTGCTG |
| Suggested primer 2: | CCCCCTGACGACTACTGTGT |