| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211266 |
| Chromosome: | chromosome_12 |
| Location: | 6198721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATACAGCCGCCGGAACACCAGGTCCAGGAA |
| Internal bar code: | GTAAAGAGTCGGACGCGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 8.31218 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 1445 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTCTTCCGGAGTGCTG |
| Suggested primer 2: | CCCCCTGACGACTACTGTGT |