| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211268 |
| Chromosome: | chromosome 7 |
| Location: | 6035105 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGTCATTTGCTGAGTGCTGCACACGCG |
| Internal bar code: | AATTCACGCAGTATCACACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.7689 |
| LEAP-Seq n confirming: | 18996 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGTTGCAGACTGTACCA |
| Suggested primer 2: | CCAGCTGTCCATACCCATCT |