| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211269 |
| Chromosome: | chromosome_1 |
| Location: | 3929278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g025600 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGCGGTGCTCACGCCGCCGCCGCCGCCCG |
| Internal bar code: | GTGCGAACTGAAGCATGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCATCCTGGACCAACT |
| Suggested primer 2: | TACACAGCTCTGCCCATCAG |