Insertion junction: LMJ.RY0402.211269_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.211269
Chromosome:chromosome 2
Location:3329440
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095090 (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGATGATTCACGGTACAGCGTCAAGGCAT
Internal bar code:CGGCGTCGAACCTCATGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:968
LEAP-Seq percent confirming:99.751
LEAP-Seq n confirming:1202
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACGGACTGCAATCAAAC
Suggested primer 2:TTTCATTCACTCGCAAGCAC