| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211276 |
| Chromosome: | chromosome 12 |
| Location: | 5577752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531700 | ADA,ADA3 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.6 - AMP deaminase / Myoadenylate deaminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCTCGGACGGTGCGCTCGAGGGGGCC |
| Internal bar code: | GCGCGCACCGGTGCCGCATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.7689 |
| LEAP-Seq n confirming: | 1727 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTTTGGTTGGCGTTAT |
| Suggested primer 2: | TTTGCTATCTTGCCAAGGCT |