| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211279 |
| Chromosome: | chromosome 1 |
| Location: | 6960801 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050200 | TNP8 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAATGTCCCGCTTCCACGCCCCTCTAGAA |
| Internal bar code: | CAGCCCGTCATGGGACCACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.828 |
| LEAP-Seq n confirming: | 2902 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCTTGGAGGTCTTGTA |
| Suggested primer 2: | GGATTGCGTGTGTACCTGTG |