| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211283 |
| Chromosome: | chromosome 3 |
| Location: | 2183015 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157450 | (1 of 1) IPR015233 - Orange carotenoid-binding protein, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGATGGAAATTCCGCCGAAACTCGTT |
| Internal bar code: | CTTGTGAGAAGTGCTTTGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 99.4852 |
| LEAP-Seq n confirming: | 1546 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTAGGCGCAAACTCAA |
| Suggested primer 2: | GGATTCCATATCGCCCCTAT |