| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211297 |
| Chromosome: | chromosome 12 |
| Location: | 765765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495600 | (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAGCAGGACTTGGCATTCAGGCACAGG |
| Internal bar code: | TTCAGCGGCGTCTCAGCGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 99.6238 |
| LEAP-Seq n confirming: | 15890 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCGACTGCATGGAGAA |
| Suggested primer 2: | AACACTTCCCAACCTGCATC |