| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211300 |
| Chromosome: | chromosome_17 |
| Location: | 5147454 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g735350 | FAP164 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGGAGGAGACAGGCGGCGACAGACACAAT |
| Internal bar code: | GAGCGATAAAAAAGTCACATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 98.9403 |
| LEAP-Seq n confirming: | 4855 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGGGACTGTGTTAGGGG |
| Suggested primer 2: | GGGTTGTGGAGCTAGTCAGC |