| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211301 |
| Chromosome: | chromosome 12 |
| Location: | 7583933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555150 | NUOB10 | (1 of 1) IPR020163 - Complex I subunit NDUFS6; NADH:ubiquinone oxidoreductase 17.8 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACATGCAGTGCGCTCCTTCTGCAGCGA |
| Internal bar code: | GGACTGAGCTCTCGCGGCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 81.9017 |
| LEAP-Seq n confirming: | 4634 |
| LEAP-Seq n nonconfirming: | 1024 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTCCCCTGCTTGTATCC |
| Suggested primer 2: | ACGTGGGCTTCATCTACCAC |