| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211311 |
| Chromosome: | chromosome 1 |
| Location: | 6943092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050050 | GUM1 | Putative guanidinoacetate N-methyltransferase; (1 of 1) 2.1.1.2 - Guanidinoacetate N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGTCCTGCCAGCGGCCCCGCAGGATGCG |
| Internal bar code: | GAGTTGCCCGACCTTGGGTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 98.9585 |
| LEAP-Seq n confirming: | 33920 |
| LEAP-Seq n nonconfirming: | 357 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGTTGTGAATGTGGGCT |
| Suggested primer 2: | AAAGCGCAAGAAAGGAAACA |