| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211316 |
| Chromosome: | chromosome 1 |
| Location: | 6056592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043000 | (1 of 1) PTHR30544//PTHR30544:SF6 - 23S RRNA METHYLTRANSFERASE // RADICAL SAM DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGACATAGGACAGACCCAGACTCCTGG |
| Internal bar code: | GGTGGACTTAGATCGCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 96.1962 |
| LEAP-Seq n confirming: | 6196 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACATTACGGCGTCTGCAC |
| Suggested primer 2: | ATCACCAGCTCCATCACCTC |