Insertion junction: LMJ.RY0402.211354_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.211354
Chromosome:chromosome 8
Location:745746
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTACAAGCTGAGAATCGAAGCAACAGGCC
Internal bar code:ATGGGATCGCTTAAGTGCGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:962
LEAP-Seq percent confirming:99.6556
LEAP-Seq n confirming:4630
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCCTGTTTCAAGGTGA
Suggested primer 2:GGTACATGTTCTCCACGGCT