| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.211369 |
| Chromosome: | chromosome_1 |
| Location: | 8019556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGCATGACCGGATGTGCTTCTTTACAGG |
| Internal bar code: | TGTTCAGGCGTGAGGCCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.459 |
| LEAP-Seq n confirming: | 1103 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTTGACTCGTCGCAAT |
| Suggested primer 2: | GGACCGCTACCTAGACACCA |